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Lesbian, gay, bisexual, and transgender LGBT rights in Queensland have advanced significantly from the late 20th century onwards, in line with progress on LGBT rights in Australia nationally. Private consensual sex between men has been legal in the state since , with lesbian sexual acts never criminalised. Same-sex couples are able to marry under federal law, enter into a civil partnership under state law or live together in an unregistered de facto relationship. Same-sex couples may become parents through adoption, foster care, altruistic surrogacy and, for lesbian couples, IVF. State anti-discrimination protections for sexuality and gender identity were introduced in and in the gay panic defence was abolished from the criminal law.
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The measurement of nucleic acid polymerase elongation rates is often done via a lengthy experimental process involving radiolabeled substrates, quenched elongation experiments, electrophoretic product separation, and band quantitation. In this work we describe an alternative real-time stopped-flow assay for obtaining kinetic parameters for elongation of extended sequences. Using poliovirus polymerase and self-priming hairpin RNA substrates with 6 to 26 nucleotide long templating regions, we demonstrate that the assay can be used to determine V max rates for elongation and apparent K m values for NTP utilization. Modeling the reaction kinetics as a series of irreversible steps allows us to numerically fit the entire time-based dataset by properly accounting for the temporal distribution of intermediate species. This enables us to determine average elongation rates over heterogeneous templating regions that mimic viral genome substrates.

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Comments (14)

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